Establishment of a cell line in vitro from the lesion of a clinical case of penis cancroid.

A cell line in vitro has been successfully established from the lesion of a clinical case of penis cancer which was histologically diagnosed as squamous cell carcinoma. The primary culture from the lesion was performed by means of explant culture method and the concomitant outgrowth of both epithelial and fibroblastic cells were observed at the early stage of the culture. But the fibroblastic cells were missed in 6 months after the culture onset and only the epithelial cells were transferred up to the present time (July, 1965) for about three years. The epithelial cells showed typical epithel shape and pavement-like arrangement to one another. As to the chromosomal structure of the present cell line, the 12 month culture showed bimodal distribution which had two modal peaks at 46 and 52 and thereafter they moved to one peak of 52 and further to 50. The result of the karyotype analysis showed the cell line contained abnormal chromo some. When the culture was implanted to the cheek pouches of the cortisone treated Syrian hamsters, the inoculation of 104 cells did not induce any nodular growth in hamster cheek pouches but the inoculation of 106 cells induced the growth in two of seven animals tested and 107 cells induced the lesion in all animals tested, respectively.